Quantification of cGMP in human plasma by isotope dilution LC-MS/MS
نویسندگان
چکیده
Background Cyclic guanosine monophospat (cGMP) is a second messenger activating intracellular protein kinases as part of the nitric oxide (NO)-cGMP pathway. Fast and reliable quantification of cGMP is therefore of major importance for scientific advancement in this field. However, until now only cumbersome immunoassays for the quantification of cGMP are available. Here we present a fast, reliable and specific method for the quantification of cGMP in humane plasma by isotope dilution liquid chromatography-tandem mass spectrometry (LC-MS/MS).
منابع مشابه
Target analyte quantification by isotope dilution LC-MS/MS directly referring to internal standard concentrations--validation for serum cortisol measurement.
BACKGROUND Isotope dilution LC-MS/MS methods used in the clinical laboratory typically involve multi-point external calibration in each analytical series. Our aim was to test the hypothesis that determination of target analyte concentrations directly derived from the relation of the target analyte peak area to the peak area of a corresponding stable isotope labelled internal standard compound [...
متن کاملLiquid chromatography–tandem mass spectrometry (LC-MS) method for the assignment of enalapril and enalaprilat in human plasma
A rapid and sensitive liquid chromatography–tandem mass spectrometry (LC-MS) method was developed for the determination of enalapril and enalaprilat in human plasma. Detection of analytes was achieved by tandem mass spectrometry with electrospray ionization (ESI) interface in positive ion mode which was operated under the multiple-reaction monitoring mode. Sample pretreatment was involved...
متن کاملLC-MS/MS quantification of sulforaphane and indole-3-carbinol metabolites in human plasma and urine after dietary intake of selenium-fortified broccoli.
This study aimed at developing a sensitive LC-MS/MS method for the quantification of sulforaphane (SFN) and indole-3-carbinol metabolites in plasma and urine after dietary intake of regular and selenium-fertilized broccoli using stable isotope dilution analysis. In a three-armed, placebo-controlled, randomized human intervention study with 76 healthy volunteers, 200 g of regular (485 μg of tota...
متن کاملA quick and Sensitive Liquid Chromatography–tandem Mass Spectrometry (LC-MS) Method for the Determination of Enalapril and Enalaprilat in Human Plasma: Application to a Bioequivalence Study
A rapid and sensitive liquid chromatography–tandem mass spectrometry (LC-MS) method for the estimation of enalapril and enalaprilat in human plasma. Detection of analytes was achieved by tandem mass spectrometry with electrospray ionization (ESI) interface in positive ion mode was operated under the multiple-reaction monitoring mode. Sample pretreatment involved in a one-step protein precipitat...
متن کاملMethod validation of clonidine hydrochloride in human plasma by LC-MS technique
A simple and sensitive high performance liquid chromatography-electrospray ionization mass spectrometry method has been evaluated for the assignment of clonidine hydrochloride in human plasma. The mobile phase composed of acetonitrile–water 60:40 (v/v), and 0.2% formic acid 20 µL of sample was chromatographically analyzed using a repacked ZORBAX-XDB-ODS C18 column (2.1 mm×30 m...
متن کامل